How to calculate volume of enzyme used? Please help =/
Favorites|Homepage
Subscriptions | sitemap
HOME > > How to calculate volume of enzyme used? Please help =/

How to calculate volume of enzyme used? Please help =/

[From: ] [author: ] [Date: 12-08-27] [Hit: ]
5mL.20μL of tyrosinase was used for the assay.The reaction was followed spectrophotometrically and a progress curve plotted from the spectrophotometric data.Using the progress curve, the initial velocity was calculated as 0.30 ΔA/min.......
A 20mM solution of dopamine is diluted 1 in 8. The diluted dopamine solution was used for a tyrosinase assay that had a total volume of 1.5mL. 20μL of tyrosinase was used for the assay. The reaction was followed spectrophotometrically and a progress curve plotted from the spectrophotometric data. Using the progress curve, the initial velocity was calculated as 0.30 ΔA/min.

The assay was repeated as above, with a different volume of tyrosinase. The initial velocity was 0.01 ΔA/s. What volume (in μL) of tyrosinase was used for the assay?

-
total volume = 1.5ml - 20µL enzyme = 1.48ml for dopamine and water
20mM x V1 = 2.5mM x 1.5ml.....V1 = 0.188ml domamine used
20µL enzyme
180µL dopamine
1.3ml water
rate = 0.3∆A/min = 0.005∆A/s

xµl enzyme
180µL dopamine
water
rate = 0.01∆A/s

rate doubles so enzyme concentration doubled, 40µl
1
keywords: help,of,How,Please,enzyme,volume,to,used,calculate,How to calculate volume of enzyme used? Please help =/
New
Hot
© 2008-2010 http://www.science-mathematics.com . Program by zplan cms. Theme by wukong .