Osmosis/Diffusion Question: You have a 5mL sample of a protein in 0.5M NaCl. You place the protein?

Osmosis/Diffusion Question: You have a 5mL sample of a protein in 0.5M NaCl. You place the protein/salt sample inside dialysis tubing and place the bag in a large beaker of distilled water. If your goal is to remove as much NaCl from the sample as possible, which would be more effective: 1. placing the dialysis bag in 4L of distilled water for 12 hours or 2. Placing the bag in 1 L of distilled water for 6 hours and then in another 1 L of fresh distilled water for another 6 hours?

Why?

Thank you!!

6 hours is plenty of time to reach diffusion equilibrium.

The NaCl in 5 ml would be diluted 200 times in 1L of water.
So it would be diluted 4 x 200 times in 4L.

But if placed in 1L of water and THEN in a second liter of water,
the NaCl would first be diluted 200 times, and then 200 times again.

And 200 x 200 is far more than 400.