You have been provided with the following solutions of a compound (mol. wt. = 200). How many mg would be contained in 50 µl?
(i) 0.2 M
(ii) 300 mM
(iii) 10% (w/v)
(iv) 5g/dL
Any help with working out would be greatly appreciated. Thanks!
(i) 0.2 M
(ii) 300 mM
(iii) 10% (w/v)
(iv) 5g/dL
Any help with working out would be greatly appreciated. Thanks!
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i.)
0.2M=moles/volume(L), so solve for moles.
Volume must be converted into L from uL
50uL*(10^-6L/1uL)=50 x 10^-6L
(50 x 10^-6L)*(0.2M)=1 x 10^-5 moles
1 x 10^-5 moles*(200g/mole)=2 x 10^-3 g*(10^3mg/1g)=2mg
ii.)
300mM=0.3M
(50 x 10^-6L)*(0.3M)=1.5 x 10^-5 moles
1.5 x 10^-5 moles*(200g/mole)=3 x 10^-3 g*(10^3mg/1g)=3mg
iii)
10%(w/v)=10g/mL
Convert volume to L
50 x 10^-6L*(10^3mL/1L)=50 x 10^-3mL
10g/mL*(50 x 10^-3mL)*(10^3mg/1g)=500mg
iv)
Convert volume to dL
dL=0.1L
50 x 10^-6L*(1dL/10^-1L)=50 x 10^-5 dL
5g/dL*(50 x 10^-5 dL)*(10^3mg/1g)=2.5mg
Edit: typo
0.2M=moles/volume(L), so solve for moles.
Volume must be converted into L from uL
50uL*(10^-6L/1uL)=50 x 10^-6L
(50 x 10^-6L)*(0.2M)=1 x 10^-5 moles
1 x 10^-5 moles*(200g/mole)=2 x 10^-3 g*(10^3mg/1g)=2mg
ii.)
300mM=0.3M
(50 x 10^-6L)*(0.3M)=1.5 x 10^-5 moles
1.5 x 10^-5 moles*(200g/mole)=3 x 10^-3 g*(10^3mg/1g)=3mg
iii)
10%(w/v)=10g/mL
Convert volume to L
50 x 10^-6L*(10^3mL/1L)=50 x 10^-3mL
10g/mL*(50 x 10^-3mL)*(10^3mg/1g)=500mg
iv)
Convert volume to dL
dL=0.1L
50 x 10^-6L*(1dL/10^-1L)=50 x 10^-5 dL
5g/dL*(50 x 10^-5 dL)*(10^3mg/1g)=2.5mg
Edit: typo
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(i) 0.2 M * 50 uL = 10 umole = 0.010 mmole,
then
0.010 mmole * 200 mg/mmole = 2 mg.
then
0.010 mmole * 200 mg/mmole = 2 mg.