just a basic question :) thanks so much!-Because DNA polymerase can only use the 3 OH as a substrate.That is the part of the nucleotide that fits in its active site.All life evolved from an ancestor that produced this specific enzyme.The 3 OH attaches to the first phosphate group on the nucleotide triphosphates breaking the pyrophosphate molecule free.This is a high energy reaction and is irreversible because the pyrophosphate breaks down into inorganic phosphates.......
I know it has something to do with hydrogen bonds...I think...just a basic question :) thanks so much!
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Because DNA polymerase can only use the 3' OH as a substrate. That is the part of the nucleotide that fits in its active site. All life evolved from an ancestor that produced this specific enzyme. The 3' OH attaches to the first phosphate group on the nucleotide triphosphates breaking the pyrophosphate molecule free. This is a high energy reaction and is irreversible because the pyrophosphate breaks down into inorganic phosphates.
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