The amount of alakaline phosphatase in a 2.5mL bottle bottle is 25000U. How many microlitres o alkaline phosphatase would you add to buffer to get 500 mL diluted solution at a conc of 300 U/L?
Can you please answer in steps so I can apply to similar questions?
Can you please answer in steps so I can apply to similar questions?
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1) 25,000 U / 2.5 ml = 10,000 U/ml
2) 300 U/L = 0.3 U/ml
3) (0.3 U/ml) / (10,000 U/ml) = 0.00003 [this is the dilution factor]
4) You want 500 ml final volume, so 500 ml * 0.00003 = 0.015 ml, or 15 µl.
5) Take 15 microliters of your alk phos stock and dilute it into a final volume of 500 ml with buffer--i.e. 15 µl alk phos + 499.985 ml buffer.
2) 300 U/L = 0.3 U/ml
3) (0.3 U/ml) / (10,000 U/ml) = 0.00003 [this is the dilution factor]
4) You want 500 ml final volume, so 500 ml * 0.00003 = 0.015 ml, or 15 µl.
5) Take 15 microliters of your alk phos stock and dilute it into a final volume of 500 ml with buffer--i.e. 15 µl alk phos + 499.985 ml buffer.