Calculating amount of enzyme needed to dilute buffer
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Calculating amount of enzyme needed to dilute buffer

[From: ] [author: ] [Date: 11-09-30] [Hit: ]
000 U/ml) = 0.4) You want 500 ml final volume, so 500 ml * 0.00003 = 0.015 ml, or 15 µl.......
The amount of alakaline phosphatase in a 2.5mL bottle bottle is 25000U. How many microlitres o alkaline phosphatase would you add to buffer to get 500 mL diluted solution at a conc of 300 U/L?

Can you please answer in steps so I can apply to similar questions?

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1) 25,000 U / 2.5 ml = 10,000 U/ml

2) 300 U/L = 0.3 U/ml

3) (0.3 U/ml) / (10,000 U/ml) = 0.00003 [this is the dilution factor]

4) You want 500 ml final volume, so 500 ml * 0.00003 = 0.015 ml, or 15 µl.

5) Take 15 microliters of your alk phos stock and dilute it into a final volume of 500 ml with buffer--i.e. 15 µl alk phos + 499.985 ml buffer.
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