We did a lab in class about dilution. We're given 3M of an unknown substance and we dilute it 4 times with 5ml of water each time. Then we used a spectrophotometer to measure absorbance of the solution. Now we have to calculate the M of each dilution and I don't know how without knowing what the substance is. Can anyone explain?
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I'll need a little more info (How much of the 3M solution did you dilute?)
We'll call the 3M solution your stock. Did you take 1 ml from the stock and dilute it with 5ml and then take 1ml of that solution and dilute it with another 5 ml? and on and on and on?
Give me that info and I'll be able to help a little better.
Sounds something a little like a standard curve. You have your absorbances and you know that your starting point is 3M (which I assume you got an Abs. on as well). So you should have 5 points of data. Once you know your dilution factor (see paragraph 2 above) you'll know the concentration of the other solutions via simple division.
ex: if you abs for the 3M soltion was .100 and in one of your samples (x) you get an abs. of .050 it would be safe to say that the concentration of x=1.5M
We'll call the 3M solution your stock. Did you take 1 ml from the stock and dilute it with 5ml and then take 1ml of that solution and dilute it with another 5 ml? and on and on and on?
Give me that info and I'll be able to help a little better.
Sounds something a little like a standard curve. You have your absorbances and you know that your starting point is 3M (which I assume you got an Abs. on as well). So you should have 5 points of data. Once you know your dilution factor (see paragraph 2 above) you'll know the concentration of the other solutions via simple division.
ex: if you abs for the 3M soltion was .100 and in one of your samples (x) you get an abs. of .050 it would be safe to say that the concentration of x=1.5M