Will it cause the incorporation of an additional amino acid into the polypeptide chain, or chain termination? OR maybe a missense conservative mutation?
Thank you very much
Thank you very much
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This situation would be expected to almost always result in chain termination; codons consist of three base pairs, these three base pairs are complimentary to anti-codons on tRNA molecules which carry the amino acids for the primary polypeptide chain synthesis. If 3bp are missing then one codon will be missing hence one amino acid will be missing from the chain resulting in a premature STOP codon; thus protein truncation.
However, a Ds element is a locus where chromosome breakage occurs, if this area of genetic code is excised it may result in extended mRNA due to the missed STOP therefore resulting in an addition of several amino acids.
Because the footprint if 3bp there wont be a non-sense mutation, a miss-sense mutation is a result of a point mutation which hasn't occurred in this situation, due to the 3bp excision, so the codon wont replace the intended amino acids with an alternate.
I think it is fair to state that termination is the most likely outcome.
However, a Ds element is a locus where chromosome breakage occurs, if this area of genetic code is excised it may result in extended mRNA due to the missed STOP therefore resulting in an addition of several amino acids.
Because the footprint if 3bp there wont be a non-sense mutation, a miss-sense mutation is a result of a point mutation which hasn't occurred in this situation, due to the 3bp excision, so the codon wont replace the intended amino acids with an alternate.
I think it is fair to state that termination is the most likely outcome.