32 mg of a FAD containing enzyme was found to contain 800 microgram of FAD. This enzyme has a native molecular weight of 64 kD and is encoded by an 872 bp ORF. What can be said regarding the protein architecture and cofactor content of this enzyme.
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Hello
the monomer has 872/3 = 290 amino acids
Molecular mass = 290*110 = 32 000 D
---> protein is a dimer.
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32 - 0.8 mg protein = 31.2 mg protein = 31.2*10^-3/64 000 moles = 4.87*10^-7 moles dimer protein
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FADH2:
molecular mass: 785.55 g/mol
800*10^-6 g FAGH2 = 800*10^-6/785.55 moles = 1.018*10^-6 moles
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1.018*10^-6 moles FADH2/ 4.87*10^-7 moles dimer protein = 2.09
---> each monomer of the dimer has 1 molecule FADH2 bound.
Regards
the monomer has 872/3 = 290 amino acids
Molecular mass = 290*110 = 32 000 D
---> protein is a dimer.
------------------
32 - 0.8 mg protein = 31.2 mg protein = 31.2*10^-3/64 000 moles = 4.87*10^-7 moles dimer protein
---------
FADH2:
molecular mass: 785.55 g/mol
800*10^-6 g FAGH2 = 800*10^-6/785.55 moles = 1.018*10^-6 moles
----------
1.018*10^-6 moles FADH2/ 4.87*10^-7 moles dimer protein = 2.09
---> each monomer of the dimer has 1 molecule FADH2 bound.
Regards