Calculating frequencies m/c full points for answer + explanation please?
I am interested in determining whether a local population of fruit flies (Drosophila melanogaster) is at Hardy-Weinberg equilibrium, so I recently visited a local orchard where I collected 100 individual flies, and took them back to the lab. There, I sequenced the DNA of each fly to determine their specific genotype at the autosomal gene locus that codes for alcohol dehydrogenase (ADH; an enzyme that is necessary for flies to metabolize and detoxify ethanol).
The results of this study revealed that there were 75 individuals that were homozygous for the dominant allele E, 10 individuals that were heterozygous, and 15 individuals that were homozygous for the recessive allele e (and are thus incapable of metabolizing and detoxifying ethanol).
What is the frequency of the wild-type phenotype (capable of metabolizing and detoxifying ethanol) in this sample?
a)0.75
b)0.8
0.9
c)It is impossible to answer this question with the information provided.
d)0.85
I am interested in determining whether a local population of fruit flies (Drosophila melanogaster) is at Hardy-Weinberg equilibrium, so I recently visited a local orchard where I collected 100 individual flies, and took them back to the lab. There, I sequenced the DNA of each fly to determine their specific genotype at the autosomal gene locus that codes for alcohol dehydrogenase (ADH; an enzyme that is necessary for flies to metabolize and detoxify ethanol).
The results of this study revealed that there were 75 individuals that were homozygous for the dominant allele E, 10 individuals that were heterozygous, and 15 individuals that were homozygous for the recessive allele e (and are thus incapable of metabolizing and detoxifying ethanol).
What is the frequency of the wild-type phenotype (capable of metabolizing and detoxifying ethanol) in this sample?
a)0.75
b)0.8
0.9
c)It is impossible to answer this question with the information provided.
d)0.85
-
So, according to the text, we know that:
75 are EE (homozygous dominant)
10 are Ee (heterozygous)
15 are ee (homozygous recessive)
Now, the allele responsible for metabolizing and detoxifying ethanol is E, not e. So that rules out the 15 fruit flies that are ee.
Next, because the 75 fruit flies that are EE, both of their alleles are E, so they can definitely metabolize and detoxify ethanol.
Because the allele to metabolize and detoxify ethanol is more dominant, it can still metabolize and detoxify even if it is paired up with a recessive allele. So Ee can do that too.
The ones that can: EE and Ee
The ones that cannot: ee
EE + Ee = 75 + 10 = 85
So the answer is 85 out of the total 100 flies, or 0.85.
75 are EE (homozygous dominant)
10 are Ee (heterozygous)
15 are ee (homozygous recessive)
Now, the allele responsible for metabolizing and detoxifying ethanol is E, not e. So that rules out the 15 fruit flies that are ee.
Next, because the 75 fruit flies that are EE, both of their alleles are E, so they can definitely metabolize and detoxify ethanol.
Because the allele to metabolize and detoxify ethanol is more dominant, it can still metabolize and detoxify even if it is paired up with a recessive allele. So Ee can do that too.
The ones that can: EE and Ee
The ones that cannot: ee
EE + Ee = 75 + 10 = 85
So the answer is 85 out of the total 100 flies, or 0.85.