What is the point of doing plasmid isolation and after you have finished the gel electrophoresis how do you confirm that you have successfully isolated the plasmid. note: we added plasmids to e. coli cells. Why are we putting it in and then taking it out. Is the e. coli dna or the plasmid suppose to be different after incubation.
Thanks for all your help.
Thanks for all your help.
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plasmid isolation confirms the successful transformation of your e.coli cells. It is not absolutely necessary, but a good practice to make sure that you do not have any contaminations.
In addition, one often wants large amounts of plasmid DNA to manipulate the genetic material, so that would be another benefit of doing this experiment.
The DNA is not much different before and after E.coli, but E.coli often adds methylation to your DNA. This is relevant, for example, when you clone PCR fragments (but I assume you don't have to worry about that)
In addition, one often wants large amounts of plasmid DNA to manipulate the genetic material, so that would be another benefit of doing this experiment.
The DNA is not much different before and after E.coli, but E.coli often adds methylation to your DNA. This is relevant, for example, when you clone PCR fragments (but I assume you don't have to worry about that)