A plasmid may have the ability to
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A plasmid may have the ability to

[From: ] [author: ] [Date: 11-05-08] [Hit: ]
The bacteria that have the plasmid and express Protein X will live and proliferate because they are also resistant to Amikacin. All bacteria that failed to accept the plasmid die because of the Amikacin. I thus have a batch of bacteria with interesting code.Hope this helps.......
replicate independentely off the chromosome
be transferred cell to cell during conjugation
carry antibiotic resisitance
be involve in any of the above processes
not be involved in any of the above processes

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The answer is all of the above.

Plasmids contain DNA that are separate from the main bacterial DNA and this is expolited in lab research when they are used as vectors. Antibiotic resistance is included into plasmids so that you can tell when bacteria have accepted and incooperated plasmids that have other codes that you are interested in.

For example I want a bacteria to express protein X. I link protein X to amikacin resistance in a plasmid. I then try to get bacteria to accept the plasmid. I then take ALL the bacteria and grow them in Amikacin. The bacteria that have the plasmid and express Protein X will live and proliferate because they are also resistant to Amikacin. All bacteria that failed to accept the plasmid die because of the Amikacin. I thus have a batch of bacteria with interesting code.

Hope this helps.
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