Can anyone who is good in their Biology/Genetics area, would be so kind to help me answer these 13 qeustions, I would be so grateful. I had 13 questions out of 100 questions that I did not know the answer to. Thank you in advance!
1) Northern blotting differs from Southern blotting in that:
A) DNA is transferred to the filter instead of RNA
B) transfer occurs from the filter to the gel
C) RNA is transferred to the filter instead of DNA
D) None of the above
2) Which of the following is not used in a PCR reaction?
A) Heat stable DNA polymerase
B) Specific primers
C) Template DNA
D) All of the above are used in PCR
3) A partial diploid provides two copies of the lac operon in a bacterium. If the bacterium's genotype is: I+P-O+Z-/I-P+O+Z+, will it be able to use lactose as a carbon source?
A) No
B) Yes
4)In the Lac operon, if glucose is present and lactose is absent:
A) cyclic AMP is high and the operon will be on
B) cyclic AMP is low and the operon will be on
C) cyclic AMP is high and the operon will be off
D) cyclic AMP is low and the operon will be off
5) If a TrpR repressor is mutated such that it can not bind tryptophan, how will the trp operon be affected?
A) The operon will always be on
B) The operon will always be off
C) There will be no effect
6) In control of the Trp operon by attenuation, which of the following results in termination of transcription?
A) Trp starvation allows quick translation through two tryptophan codons in the leader sequence.
B) Trp abundance allows for quick translation through two tryptophan codons in the leader sequence.
C) Trp abundance allows translation of the entire operon.
D) Trp starvation stalls translation at the two tryptophan codons in the leader sequence.
7) In regulation of the phage lytic cycle, abundant Cro protein represses lysogenic genes.
A) True
B) False
8) An embryonic stem cell is totipotent and an adult cell is not mainly because:
1) Northern blotting differs from Southern blotting in that:
A) DNA is transferred to the filter instead of RNA
B) transfer occurs from the filter to the gel
C) RNA is transferred to the filter instead of DNA
D) None of the above
2) Which of the following is not used in a PCR reaction?
A) Heat stable DNA polymerase
B) Specific primers
C) Template DNA
D) All of the above are used in PCR
3) A partial diploid provides two copies of the lac operon in a bacterium. If the bacterium's genotype is: I+P-O+Z-/I-P+O+Z+, will it be able to use lactose as a carbon source?
A) No
B) Yes
4)In the Lac operon, if glucose is present and lactose is absent:
A) cyclic AMP is high and the operon will be on
B) cyclic AMP is low and the operon will be on
C) cyclic AMP is high and the operon will be off
D) cyclic AMP is low and the operon will be off
5) If a TrpR repressor is mutated such that it can not bind tryptophan, how will the trp operon be affected?
A) The operon will always be on
B) The operon will always be off
C) There will be no effect
6) In control of the Trp operon by attenuation, which of the following results in termination of transcription?
A) Trp starvation allows quick translation through two tryptophan codons in the leader sequence.
B) Trp abundance allows for quick translation through two tryptophan codons in the leader sequence.
C) Trp abundance allows translation of the entire operon.
D) Trp starvation stalls translation at the two tryptophan codons in the leader sequence.
7) In regulation of the phage lytic cycle, abundant Cro protein represses lysogenic genes.
A) True
B) False
8) An embryonic stem cell is totipotent and an adult cell is not mainly because:
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