Why must heat be used with application of the primary stain during acid fast staining
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Why must heat be used with application of the primary stain during acid fast staining

[From: ] [author: ] [Date: 12-07-01] [Hit: ]
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Staining species of genus Mycobacterium, penetration of the waxy cell walls is very difficult. Heating the slide during staining helps penetrate the cell walls. Another method uses a detergent to help penetrate the cell walls.

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It sets the stain and subject on to the slide
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