a. kill bacteria
b. select for cells that cannot grow
c. make direct selection of a clone possible
d. enhance survival of the cloned cell
b. select for cells that cannot grow
c. make direct selection of a clone possible
d. enhance survival of the cloned cell
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Your answer is 'c'
Antibiotic resistance genes are often included in a engineered vector as a "marker" gene. If a bacterium is successful at taking up the desired engineered vector, it will not be subject to that specific antibiotic; thus surviving it when other bacteria who haven't taken up the desired vector and will not have the resistant gene, will be killed off. Ultimately, antibiotic selection leaves behind in culture only the desired engineered bacteria.
Do you see why that would be important?
See: Use of Antibiotic Resistance Marker Genes in ...
http://www.fda.gov/food/guidancecomplian…
Antibiotic resistance genes are often included in a engineered vector as a "marker" gene. If a bacterium is successful at taking up the desired engineered vector, it will not be subject to that specific antibiotic; thus surviving it when other bacteria who haven't taken up the desired vector and will not have the resistant gene, will be killed off. Ultimately, antibiotic selection leaves behind in culture only the desired engineered bacteria.
Do you see why that would be important?
See: Use of Antibiotic Resistance Marker Genes in ...
http://www.fda.gov/food/guidancecomplian…